Correspondence: M. J. Pérez Elías, Head of HIV Unit, transmittable Diseases Department, Hospital Ramón y Cajal, Ctra de Colmenar kilometres 9, Madrid, Spain (mjperez90
*
https://orcid.org/0000-0001-5700-5257
Navbar search Filter every Clinical transmittable Diseases every IDSA JournalsAll journals mobile Microsite search Term find
search filter all Clinical contagious Diseases all IDSA Journals all Journals search input search

Abstract

A woman with mild coronavirus condition 2019 developed cervical adenopathy, being diagnosed of Epstein-Barr virus infectious mononucleosis. Us performed good needle aspiration, and demonstrate that serious acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is discovered in lymph nodes even in mild disease along v a strong expansion that terminally distinguished effector storage CD4+ T cells, a cell populace that is practically missing in lymph nodes.

You are watching: Does corona cause swollen lymph nodes


A novel coronavirus, major acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has actually caused a worldwide pandemic that respiratory disease called coronavirus condition 2019 (COVID-19). The most constant COVID-19 symptoms incorporate mild, self-limiting respiratory tract illness up to severe progressive pneumonia, multiorgan failure, and also death <1>. Although lymphadenopathy is a usual manifestation in plenty of viral diseases, radiologic analysis has thus far concluded that lymphadenopathies room characteristically absent in most SARS-CoV-2 patients who were investigated utilizing computed tomography <2>. To date, COVID-19 has actually not been reported in superficial, palpable lymph nodes (LNs). The underlying factor for the regular lymphocytopenia observed in patients with severe condition remains unclear because it is generally not feasible come sample LNs <3>.

We report a instance of a young woman who experienced mild classical COVID-19 symptoms. After 3 weeks, she had actually a torpid development with reappearance that fever and cervical node enlargement, with singular immunophenotypic LN findings.

CASE REPORT

Clinical Presentation

In early on March 2020, a 21-year-old white woman skilled symptoms the fever, malaise, frontal headache, and cough. She was monitored by phone, and suspected COVID-19 was diagnosed. Twenty-five job later, our patience contacted us again v her household caregiver since of slim persistent fever, odynophagia, and right cervical node enlargement. Antibiotics to be prescribed without improvement. 6 days later, she attended our hospital emergency room. She had actually no relevant clinical history. On physics examination, respiratory price was 12 breaths/minute and arterial oxygen saturation was 98%. The right and left cervical submandibular regions showed palpable LNs (diameter, 2−3 cm and also Supplementary Table 1) and also a radiograph confirmed normal results, except for contempt elevated C-reactive protein (6.6 mg/L). Reverse-transcription polymerase chain reaction (RT-PCR) nasopharyngeal SARS-CoV-2 swab was positive. Purulent tonsillitis was provided over the next days, with associated breakthrough of additional brand-new lymphadenopathy and also enlargement of currently LNs. To rule out a malignant etiology or the possible involvement of SARS-CoV-2, a good needle aspiration (FNA) puncture to be performed after informed consent. Symptom persisted because that >10 days and then significantly improved. By the beginning of May, to avoid precise dates, most symptoms had actually resolved, and the LNs decreased in size.

MATERIALS and also METHODS

Molecular and also Serological Assays for Virus Detection

The main point acids were extracted native the samples (serum, node FNA, and also nasopharyngeal swab) making use of NucliSens easy-Mag platform (bioMérieux, Boston, Massachusetts). Because that SARS-CoV-2 detection, the TaqMan 2019-nCov Assay Kit (Thermo Fisher, Waltham, Massachusetts) was used, amplifying ORF1ab, S and N proteins, sequences distinctive to SARS-CoV-2. Serological or molecular tests for the diagnosis the Epstein-Barr virus (EBV), cytomegalovirus, and also neurotropic viruses to be performed v commercially obtainable kits.

FNA Cytology

FNA was performed v 4 passes making use of a 25-g needle attached to an aspiration device (Cameco, Enebyberg, Sweden). In situ Hybridization (ISH) to be performed making use of the link EBV probe ISH kit (Leica Biosystems, Wetzlar, Germany), according to the manufacturer’s instructions, in formalin-fixed and also paraffin-embedded cell blocks.

Flow Cytometry

All conjugated antibodies were derived from BD Biosciences (San Jose, California). Antitoxin were added to peripheral blood (PB) or FNA samples and also incubated for 20 minutes. Samples were obtained using a FACSCanto II circulation cytometer (BD Biosciences).

All test have an adverse controls.

RESULTS

Cytologic and Immunophenotypic FNA Analysis

Cytologically, there was numerous polymorphous lymphoid cellularity, as follows: scattered germinal center component with dendritic–lymphocytic aggregates and tangible human body macrophages, and also conspicuous plasma cells, plasmacytoid lymphocytes, and large atypical cells with an immunoblastic illustration (some the same, similar thing binucleated Reed-Sternberg cells). To rule out the neoplastic population, immunophenotypic circulation cytometry to be performed. Pan-T or pan-B markers and CD4/CD8 or κ/λ ratios were no altered. Similarly, no aberrant antigens to be present. This results said a reactive LN. The high uncertainty of acute EBV-related infectious mononucleosis was suggested by FNA cytology and confirmed by intensively confident ISH outcomes for EBV in the cabinet block FNA. Consistent with this finding, high serum famous capside antibody (VCA) immunoglobulin M levels and also no Epstein Barr nuclear antibody (EBNA) antibodies indicated a main EBV infection (Supplementary Table 1).

Molecular FNA SARS-CoV-2 Analysis

RT-PCR of one FNA diluted right cervical node sample confirmed a positive an outcome for SARS-CoV-2 (cycle threshold for SARS-CoV-2 N gene = 37).

See more: Dina Manzo Real Housewives Of New Jersey, 'Rhonj': Why Did Dina Manzo Leave The Show

FNA and PB Naive and Memory T-Cell Subset Analysis

We analyzed CD4+ and also CD8+ T-cell naive and memory phenotypes in equivalent LN and also PB samples from our patient. Results were contrasted with patients that were diagnosed previously with reactive adenopathy regarded EBV or other transmittable diseases. Every subset was gated for CD45RA and CD197 (CCR7) antigen expression. Useful subsets were characterized as naive CD45RA+CD197+, central memory CD45RA−CD197+, effector storage (TEM) CD45RA−CD197−, and also CD45RA+ effector storage RA (TEMRA) CD45RA+CD197−. Naive or main memory cells, which room the 2 key CD4+ subsets that room usually detected in common or reactive LNs that are or room not infected by EBV (Figure 1B), switched almost completely to TEM and also especially to TEMRA T cells (Figure 1A). The latter populace was virtually missing in normal or reactive lymphoid tissues. However, these transforms were much less pronounced in LN CD8+ practical subsets (Figure 1C and also 1D).